G-Trap™ Ni IDA Agarose Fast Flow

g-Trap Ni IDA琼脂糖快速流动色谱柱可直接用于基于固定金属离子亲和色谱（IMAC）的蛋白质纯化。

IMAC基于过渡金属阳离子如Ni2+、Co2+与蛋白质残基组氨酸、半胱氨酸以及某种程度上色氨酸的相互作用。在G-Trap Ni IDA琼脂糖快速流中，Ni2+使用与树脂共价结合的亚氨基二乙酸基团（IDA）固定在交联的6%琼脂糖上。 

g-Trap Ni IDA琼脂糖快速流专为纯化融合了6x组氨酸（6XHis）标签的重组蛋白而设计。

其他G-Trap IDA琼脂糖快速流动色谱柱提供1 ml和5 ml柱体积g-Trap Co IDA琼脂糖快速流动。人们可以根据需要在合适柱之间进行选择。

G-Trap Ni IDA琼脂糖快速流动树脂和柱的特性如下所示。

g-Trap Ni IDA琼脂糖快速流动树脂

Matrix: Cross-linked agarose beads, 6%

Mean particle size: 90 μm

Chelating Agent: Iminodiacetic acid

Active group: Ni2+

Metal ion density: 20-40 μmol /ml

Binding Capacity: 5-10mg His-tagged protein/ml medium

pH stability Working Range: 3-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 2-14

Recommended Flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride

Storage Conditions: 2 to 8°C, 20% Ethanol

G-Trap™ Ni IDA Agarose Fast Flow, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

G-Trap™ Ni IDA Agarose Fast Flow, 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

应用程序:

• g-Trap Ni IDA琼脂糖快速流动色谱柱可直接用于基于固定金属离子亲和色谱（IMAC）的蛋白质纯化。g-Trap Ni IDA琼脂糖快速流专为纯化融合了6x组氨酸（6XHis）标签的重组蛋白而设计。