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品牌 | 其他品牌 | 货号 | 6813S |
---|---|---|---|
供货周期 | 现货 |
cellsignal 细胞检测试剂盒 BrdU Cell Proliferation Assay Kit 货号:6813S
Figure 1.C2C12 cells wereseeded at varying density inserum free medium in a 96-well plate and incubatedovernight. Serum was addedto the plate at variousconcentrations and cells wereincubated for 24 hr Finally,10μM BrdU was added to theplate and cells were incubatedfor 4 hr.
Figure 2.Treatment of MCF10A cells with HumanEpidermal Growth Factor(hEGF) #8916 increases cellproliferation as detected byBrdU Cell Proliferation AssayKit #6813. MCF 10A cells wereseeded at 1x104 cells/well in a96-well plate and incubatedovernight. Cells were thenstarved in serum free mediumovernight. hEGF was added tothe plate and cells wereincubated for 24 hr. Finally, 10μM BrdU was added to theplate and cells were incubatedfor 4 hr.
Figure 3. jurkat cells wereseeded at 4x104 cells/well in a96-well plate and incubatedovernight. Cells were thentreated with variousconcentrations of doxorubicinfor 2 hr. Finally, 10 μM BrdUwas added to the plate andcells were incubated for 4 hr.
BrdU 细胞增殖检测试剂盒使用抗 BrdU 抗体检测细胞增殖过程中掺入细胞 DNA 的 5-溴-2'-脱氧尿嘧啶 (BrdU)。当使用含有 BrdU 的标记培养基培养细胞时,这种嘧啶类似物代替胸苷掺入增殖细胞新合成的 DNA 中。去除标记培养基后,固定细胞,并使用我们的固定/变性溶液使 DNA 变性。然后加入 BrdU 小鼠单克隆抗体以检测掺入的 BrdU(DNA 变性对于提高掺入的 BrdU 对检测抗体的可及性是必要的)。然后使用抗小鼠 IgG、HRP 连接的抗体来识别结合的检测抗体。添加 HRP 底物 TMB 以显色。显色的吸光度大小与掺入细胞中的 BrdU 的数量成正比,这是细胞增殖的直接指标。
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cellsignal 细胞检测试剂盒 BrdU Cell Proliferation Assay Kit 货号:6813S
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